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Job DescriptionP47768
Confidence100.00%DateTue Jul 17 17:05:10 BST 2012
Rank3Aligned Residues1046
% Identity55%Templated1ynjc1
SCOP infobeta and beta-prime subunits of DNA dependent RNA-polymerase beta and beta-prime subunits of DNA dependent RNA-polymerase RNA-polymerase beta
Resolution3.20

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   15....20.........30.........40... ......50.........60 .........70.........80.
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Query Sequence  RNYARISEVLELPNLIEIQTKSYEWFLRE. . . . . . . . . . GLIEMFRDISPIEDFTG. . . NLSLEFVDYRLGEPKYDLEES
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Template Conservation             
  

  





 

   
  
      
                     
 
  
 
  
 
 
 

Template Sequence  KRFGRIREVIPLPPLTEIQVESYKKALQADVPPEKRENVGIQAAFKETFPIEEGDKGKGGLVLDFLEYRIGDPPFSQDEC
Template Known Secondary structure 





SS



STTT

TTS
TT


SSTT
B


SSSSSS






SS
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   4.....10.........20.........30.........40.........50.........60.........70.........80...
 
   82.......90.........100.........110.........120.........130.........140.........150.........160.
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Query Sequence  KNRDATYAAPLRVKVRLIIKETGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDAT
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Template Sequence  REKDLTYQAPLYARLQLIHKDTGLIKEDEVFLGHLPLMTEDGSFIINGADRVIVSQIHRSPGVYFTPDPARPGRYIASII
Template Known Secondary structure  T




B

SS


B

S






SSS



SSS



S


B
TTSTT
B
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   84.....90.........100.........110.........120.........130.........140.........150.........160...
 
   162.......170.........180.........190.........200.........210.........220..... ....230.........240
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Query Sequence  IIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLRNTLEKDG. TENTEQALLEIYERL
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Template Sequence  PLPKRGPWIDLEVEASGVVTMKVNKRK. FPLVLLLRVLGYDQETLVRELSAYGDLVQGLLDEAVLAMRPEEAMVRLFTLL
Template Known Secondary structure 
STTSS



TT



TT.
TT


SSSS



TTS

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   164.....170.........180.........190 .........200.........210.........220.........230.........240..
 
   241........250.........260.........270.........280.........290.........300.........310.........320
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Query Sequence  RPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTNKKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMD
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Template Sequence  RPGDPPKKDKALAYLFGLLADPKRYDLGEAGRYKAEEKLGVGLSGRTLVRFEDGEFKDE. . . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure  SSS


STTTTTSSSSSS

S
SSSTTTTTS


SSSSB
BTTTB
B
S.....................
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   243......250.........260.........270.........280.........290.........300.
 
   321........330.........340.........350.........360.........370.........380.........390... .....
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Query Sequence  VLESNANSEVFELHGSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLSG. . IGYTD
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Template Conservation  .......................................................... 
  
  


 
  
      
Template Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . VFLPTLRYLFALTAGVPGHEVD
Template Known Secondary structure  ..........................................................STTSS

TT



Template Predicted Secondary structure  ..........................................................








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   302.......310.........320...
 
   399400.........410.........420.........430.........440.........450.........460.........470........
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Query Sequence  DIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINIRPVIASIKEFFGSSQLSQFMDQANPLAE
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Template Sequence  DIDHLGNRRIRTVGELMADQFRVGLARLARGVRERMVMGSPDTLTPAKLVNSRPLEAALREFFSRSQLSQFKDETNPLSS
Template Known Secondary structure 
TTSTTS
S
TTT

TTTS

SS
TT



SS
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   324.....330.........340.........350.........360.........370.........380.........390.........400...
 
   479480.........490.........500.........510.........520.........530.........540.........550........
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Query Sequence  LTHKRRLSALGPGGLTRERAQMEVRDVHYSHYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITD
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Template Sequence  LRHKRRISALGPGGLTRERAGFDVRDVHRTHYGRICPVETPEGANIGLITSLAAYARVDALGFIRTPYRRVKN. . GVVTE
Template Known Secondary structure  TS
SSSSS

TT

STTTS

TT
TTTB
SS


SSSSTTTB
TT

TTS
B
T..T
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   404.....410.........420.........430.........440.........450.........460.........470...... ...480.
 
   559560.........570.........580.........590.........600.........610.........620.........630........
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Query Sequence  QIDYLTADEEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATACIPFLENDDSNRALM
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Template Conservation                    
    
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Template Sequence  EVVYMTASEEDRYTIAQANTPLEGDR. IATDRVVARRRGEPVIVAPEEVEFMDVSPKQVFSLNTNLIPFLEHDDANRALM
Template Known Secondary structure 

B
SSTTTTS

SSS

BTTB.


SSTT
GGG

S
GGGGS
TTGGG
TTGGGB
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   482.......490.........500....... ..510.........520.........530.........540.........550.........560
 
   639640.........650.........660.........670.........680.........690.........700 .........710...
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Query Sequence  GANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVEHVESNEILVRRLVEENGV. . . . . EHEGELDRYPLAK
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Template Conservation     
  
           
  
                        
     
          
    
 
 

    
 
Template Sequence  GSNMQTQAVPLIRAQAPVVMTGLEERVVRDSLAALYAEEDGEVVKVDGTRIAVRYEDGRLVEHPLRRYARSNQGTAFDQR
Template Known Secondary structure  SSTTS



TT
B

S
ST
S
SSS

SS
SSS




SB

TTS

B

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   561........570.........580.........590.........600.........610.........620.........630.........640
 
   714.....720.........730.........740.........750.........760.........770.........780.........790...
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Query Sequence  FKRSNSGTCYNQRPIVAVGDVVEYNEILADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEE
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Template Sequence  PRVRVG. . . . . . . . . . . . . QRVKKGDLLADGPASEEGFLALGQNVLVAIMPFDGYNFEDAIVISEELLKRDFYTSIHIER
Template Known Secondary structure 


SS.............
B

SS

SSTT
SS

SSTT
TT
TTT
S

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   641..... ...650.........660.........670.........680.........690.........700.......
 
   794.....800.........810.........820.........830.........840.........850.........860.........870...
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Query Sequence  YESEARDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAEERLLHAIFGEKAREVRD
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Template Sequence  YEIEARDTKLGPERITRDIPHLSEAALRDLDEEGIVRIGAEVKPGDILVGRTSFKGEQEPSPEERLLRSIFGEKARDVKD
Template Known Secondary structure 

TT


B

S

TT

TTTGGGB
TTSBB
TT

TT

SB

BB







TTT




S
B
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   708.710.........720.........730.........740.........750.........760.........770.........780.......
 
   874.....880.........890.........900.........910.........920.........930.........940.........950...
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Query Sequence  TSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIVQKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPI
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Template Sequence  TSLRVPPGEGGIVVGRLRLRRGDPGVELKPGVREVVRVFVAQKRKLQVGDKLANRHGNKGVVAKILPVEDMPHLPDGTPV
Template Known Secondary structure 





TT


SSSTT



TT



TT

TT



TTSS
B
TTS
B
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   788.790.........800.........810.........820.........830.........840.........850.........860.......
 
   954.....960.........970.........980.........990.........1000.......
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Query Sequence  DIMLNPLGVPSRMNIGQVLELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEA. . . . . . . . . . . . . . . . . . . . . . . . . .
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Template Sequence  DVILNPLGVPSRMNLGQILETHLGLAGYFLGQRYISPVFDGATEPEIKELLAEAFNLYFGKRQGEGFGVDKREKEVLARA
Template Known Secondary structure  S
GGGTTTS
BTT

TTTS

T



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   868.870.........880.........890.........900.........910.........920.........930.........940.......
 
   1008.1010.........1020.........1030.........1040.........1050.........1060.........1070
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Query Sequence  . . . . . . . . . . . . . . . . . GMARDGKTVLYDGRTGEPFDNRISVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQF
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Template Sequence  EKLGLVSPGKSPEEQLKELFDLGKVVLYDGRTGEPFEGPIVVGQMFIMKLYHMVEDKMHARSTGPYSLITQQPLGGKAQF
Template Known Secondary structure  TT
S
TTS
TT

B
SSSSSB
SS

S

TTTS



SS

B
TTT
SB


SSS
Template Predicted Secondary structure 


















































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   948.950.........960.........970.........980.........990.........1000.........1010.........1020.......
 
   1071........1080.........1090.........1100.........1110.........1120.........1130.........1140.........1150
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Query Sequence  GGQRFGEMEVWALEAYGAAYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMDEQDN
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Template Sequence  GGQRFGEMEVWALEAYGAAHTLQEMLTIKSDDIEGRNAAYQAIIKGEDVPEPSVPESFRVLVKELQALALDVQTLDEKDN
Template Known Secondary structure 





TT
TTTTT
TTT






S
TTS




B
TTS
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   1028.1030.........1040.........1050.........1060.........1070.........1080.........1090.........1100.......
 
   1151.....
Predicted Secondary structure 



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Query Sequence  EIEMTD
Query Conservation        
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Template Conservation        
Template Sequence  PVDIFE
Template Known Secondary structure  B

S

Template Predicted Secondary structure 


Template SS confidence 





   1108.1110...
 
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Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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