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Job DescriptionP47768
Confidence100.00%DateTue Jul 17 17:05:10 BST 2012
Rank6Aligned Residues968
% Identity25%Templated1twfb_
SCOP infobeta and beta-prime subunits of DNA dependent RNA-polymerase beta and beta-prime subunits of DNA dependent RNA-polymerase RNA-polymerase beta
Resolution2.30

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   14.....20.........30.........40.........50.........60.........70.... .....80...
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Query Sequence  RRNYARISEVLELPNLIEIQTKSYEWFLREGLIEMFRDISPIEDFTGNLSLEFVDYRLGEP. . . . . . . . . . KYDLEESKN
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Template Sequence  EDSWAVISAFFREKGLVSQQLDSFNQFVDYTLQDIICEDSTL. . . . . . IEISFGKIYVTKPMVNESDGVTHALYPQEARL
Template Known Secondary structure  TTT
TTT
B
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   28.30.........40.........50.........60......... 70.........80.........90.........100.
 
   84.....90.........100.........110.........120.. .......130.........
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Query Sequence  RDATYAAPLRVKVRLIIKETGEVKEQEVFMGDFPLMTDT. . . . . . . . . . . . . . . . . . . . . . . . GTFVINGAERVIVSQLV
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Template Sequence  RNLTYSSGLFVDVKKRTYE. . . . . . . KVFIGRLPIMLRSKNCYLSEATESDLYKLKECPFDMGGYFIINGSEKVLIAQER
Template Known Secondary structure  TT









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TTSTTSGGGT

TT

TT



STT
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   120.........130........ .140.........150.........160.........170.........180.........190..
 
   140.........150.........160.........170......... 180........ .190.........200.......
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Query Sequence  RSPSVYFNEKIDKNGRENYDATIIPNRGAWLEYETDAKDV. . . . . . . . . . . VYVRIDRTR. KLPLTVLLRALGFSSDQEI
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Template Sequence  SAGNIVQVFKKAAPSPISHVAEIRSALEKGSRFISTLQVKLYGREGSSARTIKATLPYIKQDIPIVIIFRALGIIPDGEI
Template Known Secondary structure 
SS



TTSS


SSS




S
SS
SS


TT
SS
TT

S
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   218.220.........230.........240.........250.........260.........270.........280.........290.......
 
   208.210.........220.........230.........240.........250.........260.........270.........280.......
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Query Sequence  VDLLGDNEYLRNTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTNKKLHLKHRLFN
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Template Sequence  LEHICYDVNDWQMLE. . . . . . . . . . . MLKPCVEDGFVIQDRETALDFIGRRGTALGIK. . . . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure 


TT
...........TTT

STTS
TTS
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   298.300.........310.. .......320.........330.........340....
 
   288.290.........300.........310.........320.........330.........340.........350.........360.......
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Query Sequence  QKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELHGSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAF
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Template Sequence  . . . . . . . . . . . . . . . . . . . . . . . KEKRIQYAKDILQKEFLPHITQLEGFESRKAF. . . . . . . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure  .......................TSSTTT

SSS
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   345....350.........360.........370......
 
   368.370.........380.........390.........400.........410.........420.........430.........440.......
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Query Sequence  PDSEVKCITPADIIASMSYFFNLLSGIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQL
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Template Sequence  . . . . . . . . . . . FLGYMINRLLLCALDRKDQDDRDHFGKKRLDLAGPLLAQLFKTLFKKLTKDIFRYMQR. . . . . . TVELA
Template Known Secondary structure  ...........TTSS



TT
GGG
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   377..380.........390.........400.........410.........420.........430.... .....
 
   448.450.........460... ......470.........480.........490.........500.........510.........520...
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Query Sequence  INIRPVIASIKEFFGS. . . . SQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYSHYGRMCPIETPEGPN
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Template Sequence  INAKTITSGLKYALATGNWGAGVSQVLNRYTYSSTLSHLRRTNT. . . . . . . . . . PIAKPRQLHNTHWGLVCPAETPEGQA
Template Known Secondary structure 

T









SST
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TTS

GGGTTTB
SS


SGGG
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   448.450.........460.........470.........480.........490. ........500.........510.......
 
   524.....530.........540.........550.........560.........570.........580..... ....590.........600
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Query Sequence  IGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTADEEDSYVVAQANSKLDENGR. . . FMDDEVVCRFRGNNT
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Template Sequence  CGLVKNLSLMSCISV. GTDPMPIITFLSEWGMEP. LEDYVPHQSPDATRVFVNGVWHGVHRNPARLMETLRTLRRKGDIN
Template Known Secondary structure  BTTB
TT


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TT
.GGG

GGG
TT
TTS
TSS
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   533......540....... ..550.........560..... ....570.........580.........590.........600.........610
 
   601........610.........620...... ...630.........640.........650.........660 .........670
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Query Sequence  VMAKEKMDYMDVSPKQVVSAATACIP. FLENDDSNRALMGANMQRQAVPLMNPEAPFVGTG. . . . . . . . . MEHVAARDSG
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Template Sequence  PEVSMIRDIREKELKIFTDAGRVYRPLFIVEDDESLGHKELKVRKGHIAKLMATEYQDEYTWSSLLNEGLVEYIDAEEEE
Template Known Secondary structure  TT
TTTT

S




TTSS
S






TTST
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   611........620.........630.........640.........650.........660.........670.........680.........690
 
   671........680. ........690.........700 .........710.........720........
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Query Sequence  AAITAKHRGRV. . . . . . . . . . . . . . . . . . . EHVESNEILVRRLVEENGV. . . EHEGELDRYPLAKFKRSNSGTCYNQRPI
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Template Sequence  SILIAMQPEDLEPDVDPAKRIRVSHHATTFTHCEIHPSMILGVAASIIPFPDHNQSPRNTYQSAMGKQAMGVFLTNYNVR
Template Known Secondary structure  T

SSGGGG




TTS







S



S
GGGGS
TTGGGSSSGGGS
GGGB
B
S
TTTTT
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   700.........710.........720.........730.........740.........750.........760.........770.........
 
   729730.........740........ .750.........760.........770.........780.........790.........800
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Query Sequence  VAVGDVVEYNEILADGPSME. . . . . . . . LGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEARD
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Template Sequence  M. . . DTMANILYYPQKPLGTTRAMEYLKFRELPAGQNAIVAIACYSGYNQEDSMIMNQSSIDRGLFRSLFFRSYMDQEKK
Template Known Secondary structure 
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SSS



S
TTTTTTS




SSSTTTTTTTTT




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   789 790.........800.........810.........820.........830.........840.........850.........860.....
 
   801........810.... .....820.........830.........840.........850.........860.........870.....
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Query Sequence  TKLGPEEITRDIPN. . . . . VSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAEERLLHAIFGEKAREVRDTS
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Template Sequence  YGMSITETFEKPQRTNTLRMKHGTYDKLDDDGLIAPGVRVSGEDVIIGKTTPISSK. . . . . . . . . . . . . . . . . . . . RDAS
Template Known Secondary structure  S
TT


S



SSS
B

SS
GGG

TTSB

TT

TT







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   866...870.........880.........890.........900.........910.........920. ....
 
   876...880.........890.........900.........910.........920.........930.........940.........950.....
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Query Sequence  LRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIVQKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDI
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Template Sequence  TPLRSTENGIVDQVLVTTNQDG. . . . . . . . LKFVKVRVRTTKIPQIGDKFASRHGQKGTIGITYRREDMPFTAEGIVPDL
Template Known Secondary structure 

TT


SSS........



TT

TTS

TTTS
TTS


S
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   939940.........950.........960 .........970.........980.........990.........1000.........1010
 
   956...960.........970.........980.........990.........1000.........1010.........1020.........1030.....
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Query Sequence  MLNPLGVPSRMNIGQVLELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRISVGVM
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Template Sequence  IINPHAIPSRMTVAHLIECLLSKVAALSGNEGDASPFTDITVEGISKLLREHGYQSRGFEVMYNGHTGKKLMAQIFFGPT
Template Known Secondary structure 
GGGSTTTT

S


SSSS

TTTS
TTS

TTT

B
SS
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   1011........1020.........1030.........1040.........1050.........1060.........1070.........1080.........1090
 
   1036...1040.........1050.........1060.........1070.........1080.........1090.........1100. .......
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Query Sequence  YMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEILTYKSD. . . . DTVGRVK. . .
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Template Sequence  YYQRLRHMVDDKIHARARGP. . . . . . . . . . . . . . . . GLRFGEMERDCMIAHGAASFLKERLMEASDAFRVHICGICGLMT
Template Known Secondary structure  BSS
GGGT







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T


SS


SSS
SS
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   1091........1100.........1110 .........1120.........1130.........1140.........1150....
 
   11091110.........1120.........1130.........1140.........1150.
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Query Sequence  . . . . . . . . . . TYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMDEQDNE
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Template Sequence  VIAKLNHNQFECKGCDNKIDIYQIHIPYAAKLLFQELMAMNITPRLYTDRSRD
Template Known Secondary structure 
TTTTBTTTTBSSS

TT





S
SS

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   1171........1180.........1190.........1200.........1210.........1220...
 
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Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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