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Job DescriptionP47768
Confidence100.00%DateTue Jul 17 17:05:10 BST 2012
Rank1Aligned Residues1048
% Identity54%Templated1smyc_
SCOP infobeta and beta-prime subunits of DNA dependent RNA-polymerase beta and beta-prime subunits of DNA dependent RNA-polymerase RNA-polymerase beta
Resolution2.70

  Insertion relative to template
  Deletion relative to template
  Catalytic residue from the CSA
 
Detailed help on interpreting your alignment


   15....20.........30.........40.........50...... ...60.........70.........80.
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Query Sequence  RNYARISEVLELPNLIEIQTKSYEWFLREGLIEMFRDISPIE. . . . . . . . . . . . . DFTGNLSLEFVDYRLGEPKYDLEES
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Template Sequence  KRFGRIREVIPLPPLTEIQVESYRRALQADVPPEKRENVGIQAAFRETFPIEEEDKGKGGLVLDFLEYRLGEPPFPQDEC
Template Known Secondary structure 






S



TTT

SS
TTSS









SSS







SS
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   4.....10.........20.........30.........40.........50.........60.........70.........80...
 
   82.......90.........100.........110.........120.........130.........140.........150.........160.
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Query Sequence  KNRDATYAAPLRVKVRLIIKETGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDAT
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Template Sequence  REKDLTYQAPLYARLQLIHKDTGLIKEDEVFLGHIPLMTEDGSFIINGADRVIVSQIHRSPGVYFTPDPARPGRYIASII
Template Known Secondary structure  TT






SSS














SSS

SSS

SS



SSSSS




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   84.....90.........100.........110.........120.........130.........140.........150.........160...
 
   162.......170.........180.........190.........200.........210.........220.........230 .........240
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Query Sequence  IIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLRNTLEKDGTENTE. QALLEIYERL
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Template Conservation                    
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Template Sequence  PLPKRGPWIDLEVEPNGVVSMKVNK. RKFPLVLLLRVLGYDQETLARELGAYGELVQGLMDESVFAMRPEEALIRLFTLL
Template Known Secondary structure 
SSSSS

BTTTSSS.S


T

TSSS
GGGGGTSS
T
S
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   164.....170.........180........ .190.........200.........210.........220.........230.........240..
 
   241........250.........260.........270.........280.........290.........300.........310.........320
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Query Sequence  RPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTNKKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMD
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Template Conservation                
                       
   
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Template Sequence  RPGDPPKRDKAVAYVYGLIADPRRYDLGEAGRYKAEEKLGIRLS. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Template Known Secondary structure  SS


S

TTTTSTTTT

SSTTTSSS
S

S....................................
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   243......250.........260.........270.........280......
 
   321........330.........340.........350.........360.........370.........380.........390... .....
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Query Sequence  VLESNANSEVFELHGSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLSG. . IGYTD
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Template Conservation  ...........................................  
         
   
  
  

  
         
Template Sequence  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . GRTLARFEDGEFKDEVFLPTLRYLFALTAGVPGHEVD
Template Known Secondary structure  ...........................................TTTT


SSS



TTTSS
SS



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   287..290.........300.........310.........320...
 
   399400.........410.........420.........430.........440.........450.........460.........470........
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Query Sequence  DIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINIRPVIASIKEFFGSSQLSQFMDQANPLAE
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Template Sequence  DIDHLGNRRIRTVGELMTDQFRVGLARLARGVRERMLMGSEDSLTPAKLVNSRPLEAAIREFFSRSQLSQFKDETNPLSS
Template Known Secondary structure 
TTSTTT
S
SS

SSTTS

ST
TT


SS
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   324.....330.........340.........350.........360.........370.........380.........390.........400...
 
   479480.........490.........500.........510.........520.........530.........540.........550........
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Query Sequence  LTHKRRLSALGPGGLTRERAQMEVRDVHYSHYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITD
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Template Sequence  LRHKRRISALGPGGLTRERAGFDVRDVHRTHYGRICPVETPEGANIGLITSLAAYARVDELGFIRTPYRRVVG. . GVVTD
Template Known Secondary structure  T
S

SSSS

SS

TTTSS

TT
SSSS
SS
S
SSSSSSS

BTT

SSS
B



T..TB

S
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   404.....410.........420.........430.........440.........450.........460.........470...... ...480.
 
   559560.........570.........580.........590.........600.........610.........620.........630........
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Query Sequence  QIDYLTADEEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATACIPFLENDDSNRALM
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Template Conservation       
            
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Template Sequence  EVVYMTATEEDRYTIAQANTPLEG. NRIAAERVVARRKGEPVIVSPEEVEFMDVSPKQVFSVNTNLIPFLEHDDANRALM
Template Known Secondary structure 
B

B
TS

S
S
BSS.SBB

SS
SS

TTT




GGGGSBTTTTT
TTGGGB
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   482.......490.........500..... ....510.........520.........530.........540.........550.........560
 
   639640.........650.........660.........670.........680.........690.........700 .........710.....
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Query Sequence  GANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVEHVESNEILVRRLVEENGV. . . EHEGELDRYPLAKFK
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Template Sequence  GSNMQTQAVPLIRAQAPVVMTGLEERVVRDSLAALYAEEDGEVAKVDGNRIVVRYEDGRLVEYPLRRFYRSNQGTA. LDQ
Template Known Secondary structure  TB


SS



S
S
TT
SB

SSS



SSB

TTT

B


S


TTSB
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   561........570.........580.........590.........600.........610.........620.........630...... ...
 
   716...720.........730.........740.........750.........760.........770.........780.........790.....
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Query Sequence  RSNSGTCYNQRPIVAVGDVVEYNEILADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYE
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Template Sequence  RPRVVVG. . . . . . . . . . QRVRKGDLLADGPASENGFLALGQNVLVAIMPFDGYNFEDAIVISEELLKRDFYTSIHIERYE
Template Known Secondary structure 
B


SS..........

B
TT

SB

TTBSSSSB
SS
S

SSTT
SSTTT
S
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   640...... ...650.........660.........670.........680.........690.........700.........
 
   796...800.........810.........820.........830.........840.........850.........860.........870.....
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Query Sequence  SEARDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAEERLLHAIFGEKAREVRDTS
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Template Sequence  IEARDTKLGPERITRDIPHLSEAALRDLDEEGVVRIGAEVKPGDILVGRTSFKGESEPTPEERLLRSIFGEKARDVKDTS
Template Known Secondary structure 

TT


B

S

TTS
STTTGGGTTTSB

TT
B

TT



SSSSS

S
S




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   710.........720.........730.........740.........750.........760.........770.........780.........
 
   876...880.........890.........900.........910.........920.........930.........940.........950.....
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Query Sequence  LRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIVQKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDI
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Template Sequence  LRVPPGEGGIVVRTVRLRRGDPGVELKPGVREVVRVYVAQKRKLQVGDKLANRHGNKGVVAKILPVEDMPHLPDGTPVDV
Template Known Secondary structure 

SS



SS
SS



TT


TT

TTS

TTTS
B
SSS
B
S
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   790.........800.........810.........820.........830.........840.........850.........860.........
 
   956...960.........970.........980.........990.........1000.......
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Query Sequence  MLNPLGVPSRMNIGQVLELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEA. . . . . . . . . . . . . . . . . . . . . . . . . . . .
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Template Sequence  ILNPLGVPSRMNLGQILETHLGLAGYFLGQRYISPIFDGAKEPEIKELLAQAFEVYFGKRKGEGFGVDKREVEVLRRAEK
Template Known Secondary structure 
STTTTTTTBTTT


SSSS

TT



T
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   870.........880.........890.........900.........910.........920.........930.........940.........
 
   1008.1010.........1020.........1030.........1040.........1050.........1060.........1070..
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Query Sequence  . . . . . . . . . . . . . . . GMARDGKTVLYDGRTGEPFDNRISVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGG
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Template Sequence  LGLVTPGKTPEEQLKELFLQGKVVLYDGRTGEPIEGPIVVGQMFIMKLYHMVEDKMHARSTGPYSLITQQPLGGKAQFGG
Template Known Secondary structure  TTSS
TTS
TT

B
TTTS
B
SS
B


GGGS



SS

B
SSS

B


SSS


Template Predicted Secondary structure 


















































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   950.........960.........970.........980.........990.........1000.........1010.........1020.........
 
   1073......1080.........1090.........1100.........1110.........1120.........1130.........1140.........1150..
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Query Sequence  QRFGEMEVWALEAYGAAYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMDEQDNEI
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Template Sequence  QRFGEMEVWALEAYGAAHTLQEMLTLKSDDIEGRNAAYEAIIKGEDVPEPSVPESFRVLVKELQALALDVQTLDEKDNPV
Template Known Secondary structure 



TT
TTTTT
T








SS






SSS


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   1030.........1040.........1050.........1060.........1070.........1080.........1090.........1100.........
 
   1153...
Predicted Secondary structure 



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Query Sequence  EMTD
Query Conservation      
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Template Conservation      
Template Sequence  DIFE
Template Known Secondary structure 
SST
Template Predicted Secondary structure 

Template SS confidence 



   1110...
 
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Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
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Lawrence Kelley, Benjamin Jefferys 
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