BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (76 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_O14468 Uncharacterized protein YOR304C-A n=5 Tax=Saccha...   151   5e-36
UniRef50_Q6FQ27 Similar to uniprot|O14468 Saccharomyces cerevisi...    59   6e-08
UniRef50_A7TI14 Putative uncharacterized protein n=1 Tax=Vanderw...    50   2e-05
UniRef50_C5DYE6 ZYRO0F12408p n=1 Tax=Zygosaccharomyces rouxii Re...    45   5e-04
UniRef50_Q752N4 AFR540Cp n=1 Tax=Eremothecium gossypii RepID=Q75...    42   0.005
UniRef50_A5DHY5 Putative uncharacterized protein n=1 Tax=Pichia ...    40   0.018

>UniRef50_O14468 Uncharacterized protein YOR304C-A n=5 Tax=Saccharomyces
          cerevisiae RepID=YO304_YEAST
          Length = 76

 Score =  151 bits (382), Expect = 5e-36,   Method: Compositional matrix adjust.
 Identities = 76/76 (100%), Positives = 76/76 (100%)

Query: 1  MSTEKLEASEEPQAPLANTSETNSIKGDTENIVTVFDLANEIEKSLKDVQRQMKENDDEF 60
          MSTEKLEASEEPQAPLANTSETNSIKGDTENIVTVFDLANEIEKSLKDVQRQMKENDDEF
Sbjct: 1  MSTEKLEASEEPQAPLANTSETNSIKGDTENIVTVFDLANEIEKSLKDVQRQMKENDDEF 60

Query: 61 SRSIQAIEDKLNKMSR 76
          SRSIQAIEDKLNKMSR
Sbjct: 61 SRSIQAIEDKLNKMSR 76


>UniRef50_Q6FQ27 Similar to uniprot|O14468 Saccharomyces cerevisiae YOR304ca n=1
          Tax=Candida glabrata RepID=Q6FQ27_CANGA
          Length = 85

 Score = 58.5 bits (140), Expect = 6e-08,   Method: Compositional matrix adjust.
 Identities = 26/48 (54%), Positives = 37/48 (77%)

Query: 29 TENIVTVFDLANEIEKSLKDVQRQMKENDDEFSRSIQAIEDKLNKMSR 76
          T N++T+FD+ +EIE  LK+VQ+QM +ND +F +SI AIEDKL  + R
Sbjct: 38 TNNVITMFDIVSEIEGLLKNVQKQMTDNDAQFQKSITAIEDKLRTLQR 85


>UniRef50_A7TI14 Putative uncharacterized protein n=1 Tax=Vanderwaltozyma
          polyspora DSM 70294 RepID=A7TI14_VANPO
          Length = 77

 Score = 50.1 bits (118), Expect = 2e-05,   Method: Compositional matrix adjust.
 Identities = 21/47 (44%), Positives = 38/47 (80%)

Query: 30 ENIVTVFDLANEIEKSLKDVQRQMKENDDEFSRSIQAIEDKLNKMSR 76
          E ++TVFD+A EIE SLK++Q ++ EN++ F++++ A+E KL++M +
Sbjct: 31 EKVLTVFDVATEIETSLKELQDKLVENNEHFNKTVMAMEKKLSQMEK 77


>UniRef50_C5DYE6 ZYRO0F12408p n=1 Tax=Zygosaccharomyces rouxii RepID=C5DYE6_ZYGRO
          Length = 83

 Score = 45.4 bits (106), Expect = 5e-04,   Method: Compositional matrix adjust.
 Identities = 24/70 (34%), Positives = 44/70 (62%), Gaps = 6/70 (8%)

Query: 11 EPQAPLANTSETNSIKGDTE------NIVTVFDLANEIEKSLKDVQRQMKENDDEFSRSI 64
          E Q PL ++S  ++ K  TE      + VTVF+L +EI+K LKD++  ++ ND++F +++
Sbjct: 13 EEQTPLTSSSSASTGKTTTEQDDKQGSTVTVFELTSEIKKCLKDLEDSIQSNDEKFEKTM 72

Query: 65 QAIEDKLNKM 74
          +  E K+  +
Sbjct: 73 KRFESKIKTL 82


>UniRef50_Q752N4 AFR540Cp n=1 Tax=Eremothecium gossypii RepID=Q752N4_ASHGO
          Length = 72

 Score = 42.4 bits (98), Expect = 0.005,   Method: Compositional matrix adjust.
 Identities = 18/47 (38%), Positives = 34/47 (72%)

Query: 28 DTENIVTVFDLANEIEKSLKDVQRQMKENDDEFSRSIQAIEDKLNKM 74
          + E IVTVFDLA+EIE+SL      +++N+ +F ++++ I ++L ++
Sbjct: 24 NAEKIVTVFDLASEIEQSLNQALEHVEKNEAQFQQTLKDIHERLKRL 70


>UniRef50_A5DHY5 Putative uncharacterized protein n=1 Tax=Pichia guilliermondii
          RepID=A5DHY5_PICGU
          Length = 104

 Score = 40.4 bits (93), Expect = 0.018,   Method: Compositional matrix adjust.
 Identities = 24/69 (34%), Positives = 36/69 (52%), Gaps = 2/69 (2%)

Query: 8  ASEEPQAPLANTSET--NSIKGDTENIVTVFDLANEIEKSLKDVQRQMKENDDEFSRSIQ 65
          AS E Q P+    +     I     N V+VFDLA EIE  L  + +++K +DDE + +I 
Sbjct: 21 ASVEHQTPVVRMEDAAEGDISFSAGNAVSVFDLAAEIESKLAKIGQKIKSHDDELNSAID 80

Query: 66 AIEDKLNKM 74
           + D L  +
Sbjct: 81 QVLDSLEAL 89


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_O14468 Uncharacterized protein YOR304C-A n=5 Tax=Saccha...    81   1e-14
UniRef50_Q6FQ27 Similar to uniprot|O14468 Saccharomyces cerevisi...    57   1e-07
UniRef50_C5DYE6 ZYRO0F12408p n=1 Tax=Zygosaccharomyces rouxii Re...    52   5e-06
UniRef50_A7TI14 Putative uncharacterized protein n=1 Tax=Vanderw...    49   3e-05

Sequences not found previously or not previously below threshold:

UniRef50_Q752N4 AFR540Cp n=1 Tax=Eremothecium gossypii RepID=Q75...    38   0.068


CONVERGED!
>UniRef50_O14468 Uncharacterized protein YOR304C-A n=5 Tax=Saccharomyces
          cerevisiae RepID=YO304_YEAST
          Length = 76

 Score = 80.7 bits (197), Expect = 1e-14,   Method: Composition-based stats.
 Identities = 76/76 (100%), Positives = 76/76 (100%)

Query: 1  MSTEKLEASEEPQAPLANTSETNSIKGDTENIVTVFDLANEIEKSLKDVQRQMKENDDEF 60
          MSTEKLEASEEPQAPLANTSETNSIKGDTENIVTVFDLANEIEKSLKDVQRQMKENDDEF
Sbjct: 1  MSTEKLEASEEPQAPLANTSETNSIKGDTENIVTVFDLANEIEKSLKDVQRQMKENDDEF 60

Query: 61 SRSIQAIEDKLNKMSR 76
          SRSIQAIEDKLNKMSR
Sbjct: 61 SRSIQAIEDKLNKMSR 76


>UniRef50_Q6FQ27 Similar to uniprot|O14468 Saccharomyces cerevisiae YOR304ca n=1
          Tax=Candida glabrata RepID=Q6FQ27_CANGA
          Length = 85

 Score = 57.2 bits (136), Expect = 1e-07,   Method: Composition-based stats.
 Identities = 26/48 (54%), Positives = 37/48 (77%)

Query: 29 TENIVTVFDLANEIEKSLKDVQRQMKENDDEFSRSIQAIEDKLNKMSR 76
          T N++T+FD+ +EIE  LK+VQ+QM +ND +F +SI AIEDKL  + R
Sbjct: 38 TNNVITMFDIVSEIEGLLKNVQKQMTDNDAQFQKSITAIEDKLRTLQR 85


>UniRef50_C5DYE6 ZYRO0F12408p n=1 Tax=Zygosaccharomyces rouxii RepID=C5DYE6_ZYGRO
          Length = 83

 Score = 52.2 bits (123), Expect = 5e-06,   Method: Composition-based stats.
 Identities = 24/74 (32%), Positives = 46/74 (62%), Gaps = 6/74 (8%)

Query: 8  ASEEPQAPLANTSETNSIKGDTE------NIVTVFDLANEIEKSLKDVQRQMKENDDEFS 61
          ++ E Q PL ++S  ++ K  TE      + VTVF+L +EI+K LKD++  ++ ND++F 
Sbjct: 10 SNREEQTPLTSSSSASTGKTTTEQDDKQGSTVTVFELTSEIKKCLKDLEDSIQSNDEKFE 69

Query: 62 RSIQAIEDKLNKMS 75
          ++++  E K+  + 
Sbjct: 70 KTMKRFESKIKTLE 83


>UniRef50_A7TI14 Putative uncharacterized protein n=1 Tax=Vanderwaltozyma
          polyspora DSM 70294 RepID=A7TI14_VANPO
          Length = 77

 Score = 49.5 bits (116), Expect = 3e-05,   Method: Composition-based stats.
 Identities = 24/67 (35%), Positives = 42/67 (62%)

Query: 10 EEPQAPLANTSETNSIKGDTENIVTVFDLANEIEKSLKDVQRQMKENDDEFSRSIQAIED 69
          EE         E  +     E ++TVFD+A EIE SLK++Q ++ EN++ F++++ A+E 
Sbjct: 11 EEKPLTTPGNDENANDSTSEEKVLTVFDVATEIETSLKELQDKLVENNEHFNKTVMAMEK 70

Query: 70 KLNKMSR 76
          KL++M +
Sbjct: 71 KLSQMEK 77


>UniRef50_Q752N4 AFR540Cp n=1 Tax=Eremothecium gossypii RepID=Q752N4_ASHGO
          Length = 72

 Score = 38.3 bits (87), Expect = 0.068,   Method: Composition-based stats.
 Identities = 18/59 (30%), Positives = 38/59 (64%)

Query: 18 NTSETNSIKGDTENIVTVFDLANEIEKSLKDVQRQMKENDDEFSRSIQAIEDKLNKMSR 76
          +     + + + E IVTVFDLA+EIE+SL      +++N+ +F ++++ I ++L ++ +
Sbjct: 14 SAGSGEAAEQNAEKIVTVFDLASEIEQSLNQALEHVEKNEAQFQQTLKDIHERLKRLEQ 72


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.288    0.117    0.260 

Lambda     K      H
   0.267   0.0359    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 184,427,007
Number of Sequences: 3077464
Number of extensions: 4874900
Number of successful extensions: 29183
Number of sequences better than 1.0e-01: 7
Number of HSP's better than  0.1 without gapping: 13
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 29169
Number of HSP's gapped (non-prelim): 14
length of query: 76
length of database: 1,040,396,356
effective HSP length: 47
effective length of query: 29
effective length of database: 895,755,548
effective search space: 25976910892
effective search space used: 25976910892
T: 11
A: 40
X1: 16 ( 6.7 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (21.0 bits)
S2: 86 (37.9 bits)
