HEADER TRANSCRIPTION REGULATION 13-JAN-94 2BOP 2BOP 2 COMPND BOVINE PAPILLOMAVIRUS-1 E2 (DNA-BINDING DOMAIN) 2BOP 3 COMPND 2 (RESIDUES 325 - 410) COMPLEXED WITH DNA 2BOP 4 SOURCE BOVINE PAPILLOMAVIRUS-1 GENE FRAGMENT RECOMBINANT FORM 2BOP 5 SOURCE 2 EXPRESSED IN (ESCHERICHIA COLI) 2BOP 6 AUTHOR R.S.HEGDE,S.R.GROSSMAN,L.A.LAIMINS,P.B.SIGLER 2BOP 7 REVDAT 1 31-JAN-94 2BOP 0 2BOP 8 JRNL AUTH R.S.HEGDE,S.R.GROSSMAN,L.A.LAIMINS,P.B.SIGLER 2BOP 9 JRNL TITL CRYSTAL STRUCTURE AT 1.7 ANGSTROMS OF THE BOVINE 2BOP 10 JRNL TITL 2 PAPILLOMAVIRUS-1 E2 DNA-BINDING DOMAIN BOUND TO 2BOP 11 JRNL TITL 3 ITS DNA TARGET 2BOP 12 JRNL REF NATURE V. 359 505 1992 2BOP 13 JRNL REFN ASTM NATUAS UK ISSN 0028-0836 006 2BOP 14 REMARK 1 2BOP 15 REMARK 2 2BOP 16 REMARK 2 RESOLUTION. 1.7 ANGSTROMS. 2BOP 17 REMARK 3 2BOP 18 REMARK 3 REFINEMENT. 2BOP 19 REMARK 3 PROGRAM 1 PROLSQ 2BOP 20 REMARK 3 AUTHORS 1 KONNERT,HENDRICKSON 2BOP 21 REMARK 3 PROGRAM 2 NUCLSQ 2BOP 22 REMARK 3 AUTHORS 2 WESTHOF,DUMAS,MORAS 2BOP 23 REMARK 3 PROGRAM 3 X-PLOR 2BOP 24 REMARK 3 AUTHORS 3 BRUNGER 2BOP 25 REMARK 3 R VALUE 0.2007 2BOP 26 REMARK 3 RMSD BOND DISTANCES 0.02 ANGSTROMS 2BOP 27 REMARK 3 2BOP 28 REMARK 3 RESOLUTION RANGE 6.0 - 1.7 ANGSTROMS 2BOP 29 REMARK 4 2BOP 30 REMARK 4 THE PROTEIN FORMS A DIMER. THE DUPLEX DNA COMPLEX 2BOP 31 REMARK 4 OCCUPIES THE CRYSTALLOGRAPHIC TWO-FOLD. THE FOLLOWING 2BOP 32 REMARK 4 TRANSFORMATION CAN BE USED TO GENERATE THE DIMER FROM 2BOP 33 REMARK 4 THE COORDINATES GIVEN: 2BOP 34 REMARK 4 2BOP 35 REMARK 4 MTRIX1 -0.472531 -0.881331 0.000000 220.77284 2BOP 36 REMARK 4 MTRIX2 -0.881298 0.472531 0.000000 132.13081 2BOP 37 REMARK 4 MTRIX3 0.000000 0.000000 -1.000000 53.91998 2BOP 38 REMARK 5 2BOP 39 REMARK 5 THE PROTEIN FORMS A DIMERIC BARREL. THE FOUR STRANDS OF 2BOP 40 REMARK 5 EACH BETA SHEET DESCRIBED BELOW COMPRISE A HALF BARREL. 2BOP 41 REMARK 5 THE OTHER HALF IS GENERATED BY A CRYSTALLOGRAPHIC TWO-FOLD. 2BOP 42 SEQRES 1 A 85 SER CYS PHE ALA LEU ILE SER GLY THR ALA ASN GLN VAL 2BOP 43 SEQRES 2 A 85 LYS CYS TYR ARG PHE ARG VAL LYS LYS ASN HIS ARG HIS 2BOP 44 SEQRES 3 A 85 ARG TYR GLU ASN CYS THR THR THR TRP PHE THR VAL ALA 2BOP 45 SEQRES 4 A 85 ASP ASN GLY ALA GLU ARG GLN GLY GLN ALA GLN ILE LEU 2BOP 46 SEQRES 5 A 85 ILE THR PHE GLY SER PRO SER GLN ARG GLN ASP PHE LEU 2BOP 47 SEQRES 6 A 85 LYS HIS VAL PRO LEU PRO PRO GLY MET ASN ILE SER GLY 2BOP 48 SEQRES 7 A 85 PHE THR ALA SER LEU ASP PHE 2BOP 49 SEQRES 1 B 17 C C G A C C G A C G T C G 2BOP 50 SEQRES 2 B 17 G T C G 2BOP 51 FTNOTE 1 2BOP 52 FTNOTE 1 YTTERBIUM WAS USED IN THE CRYSTALLIZATION AND PHASING OF 2BOP 53 FTNOTE 1 THIS STRUCTURE. IT HAS NO ROLE IN DNA-BINDING OR DNA 2BOP 54 FTNOTE 1 SEQUENCE RECOGNITION. 2BOP 55 HET YB 499 1 YTTERBIUM ION 2BOP 56 HET YB 500 1 YTTERBIUM ION 2BOP 57 FORMUL 3 YB 2(YB1 +++) 2BOP 58 FORMUL 4 HOH *121(H2 O1) 2BOP 59 HELIX 1 A1 THR A 334 HIS A 349 1 2BOP 60 HELIX 2 A2 SER A 382 VAL A 393 1 2BOP 61 SHEET 1 B 4 TYR A 353 ASP A 365 0 2BOP 62 SHEET 2 B 4 GLU A 369 GLY A 381 -1 2BOP 63 SHEET 3 B 4 SER A 326 THR A 334 -1 2BOP 64 SHEET 4 B 4 MET A 399 PHE A 410 -1 2BOP 65 TURN 1 TR1 HIS A 349 ARG A 352 B-BEND 2BOP 66 TURN 2 TR2 ASN A 366 GLU A 369 B-BEND 2BOP 67 TURN 3 TR3 PRO A 396 MET A 399 B-BEND 2BOP 68 CRYST1 64.600 64.600 64.600 61.80 61.80 61.80 R 3 2 6 2BOP 69 ORIGX1 1.000000 0.000000 0.000000 0.00000 2BOP 70 ORIGX2 0.000000 1.000000 0.000000 0.00000 2BOP 71 ORIGX3 0.000000 0.000000 1.000000 0.00000 2BOP 72 SCALE1 0.015480 -0.008300 -0.005951 0.00000 2BOP 73 SCALE2 0.000000 0.017565 -0.005951 0.00000 2BOP 74 SCALE3 0.000000 0.000000 0.018546 0.00000 2BOP 75