Alignment of thymidine_kinase with 1reqA2

Alignment of thymidine_kinase with 1reqA2

PSSM Score Key
-10																			10

Bad																			Good

thymid_PSS	Predicted Secondary Structure for your Sequence
thymid_Seq	Your Sequence
1reqA2_Seq	Library Sequence
1reqA2_SS	Known Secondary Structure of the Library Sequence (Assigned by STRIDE)
CORE	A measure of the burial and number of contacts made by a residue. (9 very buried/making many contacts, 0 not buried/making few contacts

thymid_PSS

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

E

E

E

E

E

E

E

E

E

C

C

C

C

H

H

H

H

H

H

H

H

H

H

H

H

H

H

C

C

C

E

E

thymid_Seq

M

S

C

I

N

L

P

T

V

L

P

G

S

P

S

K

T

R

G

Q

I

Q

V

I

L

G

P

M

F

S

G

K

S

T

E

L

M

R

R

V

R

R

F

Q

I

A

Q

Y

K

C

-------

-

-

-

-

+

-

-

V

-

-

-

+

-

T

-

-

-

E

L

-

+

-

-

-

+

-

+

+

1reqA2_Seq

A

Q

I

R

T

I

S

G

V

Y

S

K

E

V

K

N

T

P

.

.

.

.

.

.

.

.

.

.

E

V

E

E

A

R

E

L

V

E

E

F

E

Q

A

E

G

R

R

P

R

I

1reqA2_SS

C

C

C

C

C

C

C

C

H

H

H

H

H

C

C

C

C

H

.

.

.

.

.

.

.

.

.

.

H

H

H

H

H

H

H

H

H

H

H

H

H

H

H

H

C

C

C

C

E

E

CORE

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

.

.

.

.

.

.

.

.

.

.

0

0

0

0

2

0

0

0

2

0

0

0

0

0

0

0

0

0

0

1

0

3

thymid_PSS

E

E

E

E

E

C

C

C

C

C

C

C

C

C

E

E

E

E

C

C

C

C

C

C

C

C

C

C

C

C

H

H

H

H

H

H

H

H

C

C

E

E

E

E

E

E

E

C

C

C

thymid_Seq

L

V

I

K

Y

A

K

D

T

R

Y

S

S

S

F

C

T

H

D

R

N

T

M

E

A

L

P

A

C

L

L

R

D

V

A

Q

E

A

L

G

V

A

V

I

G

I

D

E

G

Q

-------

L

+

-

K

-

D

-

H

D

R

-

-

-

+

-

+

+

A

-

L

G

-

-

V

D

G

-

1reqA2_Seq

L

L

A

K

M

G

Q

D

.

.

.

.

.

.

.

.

G

H

D

R

G

.

.

.

.

.

.

.

Q

K

V

I

A

T

A

Y

A

D

L

G

F

D

V

.

.

.

D

V

G

P

1reqA2_SS

E

E

E

C

E

C

C

C

.

.

.

.

.

.

.

.

C

C

H

H

H

.

.

.

.

.

.

.

H

H

H

H

H

H

H

H

H

H

H

C

C

E

E

.

.

.

E

E

C

C

CORE

5

1

6

0

4

0

0

0

.

.

.

.

.

.

.

.

0

0

0

0

0

.

.

.

.

.

.

.

0

0

0

0

1

0

0

1

0

0

2

0

2

0

1

.

.

.

0

0

1

0

thymid_PSS

C

C

C

H

H

H

H

H

H

H

H

H

H

H

C

C

C

E

E

E

.

E

E

C

C

C

C

C

H

H

H

C

C

C

C

C

H

H

H

H

H

H

H

H

H

H

H

H

H

C

thymid_Seq

F

F

P

D

I

M

E

F

C

E

A

M

A

N

A

G

K

T

V

I

.

V

A

A

L

D

G

T

F

Q

R

K

P

F

G

A

I

L

N

L

V

P

L

A

E

S

V

V

K

L

-------

-

F

-

-

-

E

+

-

+

+

-

-

A

+

-

-

V

+

V

L

-

G

G

-

-

-

-

+

-

L

+

+

-

-

K

L

1reqA2_Seq

L

F

Q

T

P

E

E

T

A

R

Q

A

V

E

A

D

V

H

V

V

G

V

S

S

L

A

G

.

.

.

.

.

.

.

G

H

L

T

L

V

P

A

L

R

K

E

L

D

K

L

1reqA2_SS

C

C

E

C

H

H

H

H

H

H

H

H

H

H

H

C

C

C

E

E

E

E

E

E

C

C

C

.

.

.

.

.

.

.

H

H

H

H

H

H

H

H

H

H

H

H

H

H

H

H

CORE

0

0

0

0

1

0

0

4

3

0

0

8

2

0

0

0

3

0

2

9

2

7

0

1

0

0

0

.

.

.

.

.

.

.

0

0

0

0

1

6

0

0

6

0

0

0

6

0

0

1

thymid_PSS		C	H	H	H	H	H	H	C	C	C		C	C	E	E	E	E	E	C	C	C		C	E	E	E	E	E	C	C	C	C		C	C	H	H	H	H	H	H	H	C		C	C	C	C	C	C	C	C	C	C
thymid_Seq		T	A	V	C	M	E	C	F	R	E		A	A	Y	T	K	R	L	G	T	E		K	E	V	E	V	I	G	G	A	D		K	Y	H	S	V	C	R	L	C	Y		F	K	K	A	S	G	Q	P	A	G
-------		-																			-	+			-	-	-	+	+	G	G																	-	-	+	+	Q	-	+	-
1reqA2_Seq		G	.	.	.	.	.	.	.	.	.		.	.	.	.	.	.	.	.	R	P		D	I	L	I	T	V	G	G	.	.		.	.	.	.	.	.	.	.	.	.		.	.	V	I	P	E	Q	D	F	D
1reqA2_SS		C	.	.	.	.	.	.	.	.	.		.	.	.	.	.	.	.	.	C	C		C	C	E	E	E	E	E	E	.	.		.	.	.	.	.	.	.	.	.	.		.	.	C	C	C	C	C	H	H	H
CORE		0	.	.	.	.	.	.	.	.	.		.	.	.	.	.	.	.	.	0	0		0	5	1	6	1	5	0	0	.	.		.	.	.	.	.	.	.	.	.	.		.	.	0	1	0	0	0	0	0	0

thymid_PSS

C

C

C

C

C

C

C

C

C

C

C

C

C

C

H

H

H

H

H

H

H

H

C

C

C

H

H

H

H

C

C

C

C

C

C

thymid_Seq

P

D

N

K

E

N

C

P

V

P

G

K

P

G

E

A

V

A

A

R

K

L

F

A

P

Q

Q

I

L

Q

C

S

P

A

N

-------

-

-

K

-

-

+

-

P

G

-

-

+

-

-

-

-

-

+

L

-

-

S

-

-

-

1reqA2_Seq

E

L

R

K

D

G

A

V

E

I

Y

T

P

G

T

V

I

P

E

S

A

I

.

S

L

V

K

K

L

R

A

S

L

D

A

1reqA2_SS

H

H

H

H

H

C

E

E

E

E

E

C

C

C

C

C

H

H

H

H

H

H

.

H

H

H

H

H

H

H

H

H

H

H

C

CORE

0

4

0

0

0

1

4

0

0

2

0

0

0

0

0

0

1

0

0

0

2

0

.

0

2

1

0

0

1

0

0

0

0

0

0