Alignment of thymidine_kinase with 1eft-3

Alignment of thymidine_kinase with 1eft-3

PSSM Score Key
-10																			10

Bad																			Good

thymid_PSS	Predicted Secondary Structure for your Sequence
thymid_Seq	Your Sequence
1eft-3_Seq	Library Sequence
1eft-3_SS	Known Secondary Structure of the Library Sequence (Assigned by STRIDE)
CORE	A measure of the burial and number of contacts made by a residue. (9 very buried/making many contacts, 0 not buried/making few contacts

thymid_PSS

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

E

E

E

E

E

.

E

E

E

E

C

C

C

C

H

H

H

H

H

H

H

H

H

H

H

H

H

H

C

C

C

E

thymid_Seq

M

S

C

I

N

L

P

T

V

L

P

G

S

P

S

K

T

R

G

Q

I

Q

V

.

I

L

G

P

M

F

S

G

K

S

T

E

L

M

R

R

V

R

R

F

Q

I

A

Q

Y

K

-------

+

G

-

-

+

T

-

-

V

+

+

G

-

-

G

K

+

T

-

+

+

+

-

+

-

-

-

+

1eft-3_Seq

A

K

.

.

.

.

.

.

.

.

.

G

E

F

I

R

T

K

P

H

V

N

V

G

T

I

G

H

V

D

H

G

K

T

T

L

T

A

A

L

T

F

V

T

A

A

E

N

P

N

1eft-3_SS

C

C

.

.

.

.

.

.

.

.

.

C

E

C

C

C

C

C

C

E

E

E

E

E

E

E

C

C

C

C

C

C

H

H

H

H

H

H

H

H

H

H

H

H

H

H

H

C

C

C

CORE

0

0

.

.

.

.

.

.

.

.

.

0

0

0

0

0

0

0

0

0

0

0

4

3

3

5

1

0

0

0

0

2

0

0

1

9

1

1

7

5

0

0

3

0

0

0

0

0

0

0

thymid_PSS		E	E	E	.	.	.	.	.	.	.		.	.	.	.	.	.	.	.	.	.		.	.	.	.	.	.	E	E	E	C		C	C	C	C	C	C	C	C	E	E		E	E	C	C	C	C	C	C	C	C
thymid_Seq		C	L	V	.	.	.	.	.	.	.		.	.	.	.	.	.	.	.	.	.		.	.	.	.	.	.	I	K	Y	A		K	D	T	R	Y	S	S	S	F	C		T	H	D	R	N	T	M	E	A	L
-------			-	V																										+	+	Y			-			R									H
1eft-3_Seq		V	E	V	K	D	Y	G	D	I	D		K	A	P	E	E	R	A	R	G	I		T	I	N	T	A	H	V	E	Y	E		T	A	K	R	H	.	.	.	.	Y		S	H	.	.	.	.	.	.	.	V
1eft-3_SS		C	C	C	C	C	H	H	H	C	C		C	C	H	H	H	H	H	H	C	C		C	C	C	C	E	E	E	E	E	E		C	C	C	C	E	.	.	.	.	E		E	E	.	.	.	.	.	.	.	E
CORE		1	0	0	0	0	0	0	0	1	0		0	0	0	0	0	0	0	0	0	0		0	0	0	0	0	0	1	0	1	0		0	0	0	0	0	.	.	.	.	0		0	1	.	.	.	.	.	.	.	1

thymid_PSS

C

C

C

C

.

H

H

H

H

H

H

H

H

C

.

.

.

.

.

C

E

E

E

E

E

E

E

C

C

C

C

.

C

C

H

H

H

H

H

H

H

H

H

H

H

C

C

C

E

E

thymid_Seq

P

A

C

L

.

L

R

D

V

A

Q

E

A

L

.

.

.

.

.

G

V

A

V

I

G

I

D

E

G

Q

F

.

F

P

D

I

M

E

F

C

E

A

M

A

N

A

G

K

T

V

-------

-

-

-

-

+

+

+

-

+

-

-

+

A

+

-

+

P

+

E

-

+

+

-

G

-

1eft-3_Seq

D

C

P

G

H

A

D

Y

I

K

N

M

I

T

G

A

A

Q

M

D

G

A

I

L

V

V

S

A

A

D

G

P

M

P

Q

T

R

E

H

I

L

L

A

R

Q

V

G

V

P

Y

1eft-3_SS

E

C

C

C

C

H

H

H

H

H

H

H

H

H

H

H

H

C

C

C

E

E

E

E

E

E

E

C

C

C

C

C

C

H

H

H

H

H

H

H

H

H

H

H

H

C

C

C

C

E

CORE

0

2

0

0

0

0

0

0

0

0

0

1

0

0

0

2

0

0

2

0

2

5

8

7

4

3

0

1

0

0

1

1

0

0

0

2

0

0

0

4

0

0

3

0

0

0

0

3

0

0

thymid_PSS

E

E

E

C

C

C

C

C

H

H

H

C

.

.

C

C

C

C

H

H

H

H

H

H

H

H

H

H

H

H

H

C

C

H

H

H

H

H

H

C

C

C

C

C

E

E

E

E

E

C

thymid_Seq

I

V

A

A

L

D

G

T

F

Q

R

K

.

.

P

F

G

A

I

L

N

L

V

P

L

A

E

S

V

V

K

L

T

A

V

C

M

E

C

F

R

E

A

A

Y

T

K

R

L

G

-------

I

V

F

-

-

K

-

+

+

+

-

-

L

-

L

+

E

-

-

V

+

+

-

-

+

-

1eft-3_Seq

I

V

V

.

.

.

.

.

F

M

N

K

V

D

M

V

D

D

.

P

E

L

L

D

L

V

E

M

E

V

R

.

.

.

.

.

.

D

L

L

N

Q

Y

E

F

.

.

.

.

.

1eft-3_SS

E

E

E

.

.

.

.

.

E

E

E

C

H

H

H

C

C

C

.

C

H

H

H

H

H

H

H

H

H

H

H

.

.

.

.

.

.

H

H

H

H

H

C

C

C

.

.

.

.

.

CORE

7

4

5

.

.

.

.

.

7

1

0

0

1

0

0

1

0

0

.

0

0

0

1

0

0

1

0

0

0

4

0

.

.

.

.

.

.

0

1

6

0

0

0

0

2

.

.

.

.

.

thymid_PSS

C

C

C

E

E

E

E

E

C

C

C

C

C

C

H

H

H

H

H

H

H

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

C

H

H

H

H

thymid_Seq

T

E

K

E

V

E

V

I

G

G

A

D

K

Y

H

S

V

C

R

L

C

Y

F

K

K

A

S

G

Q

P

A

G

P

D

N

K

E

N

C

P

V

P

G

K

P

G

E

A

V

A

-------

-

+

E

V

V

I

G

-

+

+

-

-

-

+

P

-

-

K

-

G

E

-

-

1eft-3_Seq

P

G

D

E

V

P

V

I

R

G

S

A

L

.

L

A

L

E

E

M

H

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

K

N

P

K

.

T

K

R

G

E

N

E

W

1eft-3_SS

C

C

C

C

C

C

E

E

E

C

C

H

H

.

H

H

H

H

H

H

H

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

H

C

C

C

.

C

C

C

C

C

C

H

H

CORE

0

1

0

0

4

1

1

4

0

1

0

9

0

.

0

5

2

0

0

1

0

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

0

0

0

0

.

0

0

0

0

0

0

0

0

thymid_PSS

H

H

H

H

C

C

C

H

H

H

H

C

C

C

C

C

C

thymid_Seq

A

R

K

L

F

A

P

Q

Q

I

L

Q

C

S

P

A

N

-------

-

K

+

-

-

-

+

-

+

-

-

-

-

1eft-3_Seq

V

D

K

I

W

E

L

L

D

A

I

D

E

Y

I

P

T

1eft-3_SS

H

H

H

H

H

H

H

H

H

H

H

H

H

H

C

C

C

CORE

2

0

0

8

0

0

7

2

0

2

3

0

0

0

2

0

0