Phyre 2 Results for G5EC32

Email	l.a.kelley@imperial.ac.uk
Description	G5EC32
Date	Sat Apr 13 13:16:31 BST 2013
Unique Job ID	78d7a22663c0beb3

Summary

Top model

Image coloured by rainbow N → C terminus

Model dimensions (Å):

X:62.819 Y:57.012 Z:51.119

Model (left) based on template c1griA_

Top template information

PDB header:signal transduction adaptor
Chain: A: PDB Molecule:growth factor bound protein 2;
PDBTitle: grb2

Confidence and coverage

Confidence:

100.0%

Coverage:

19%

188 residues ( 19% of your sequence) have been modelled with 100.0% confidence by the single highest scoring template.

Additional confident templates have been detected (see Domain analysis) which cover other regions of your sequence.

277 residues ( 28%) could be modelled at >90% confidence using multiple-templates.

You may wish to try resubmitting your sequence in "intensive" mode to model more of your sequence.

Warning: 72% of your sequence is predicted disordered. Disordered regions cannot be meaningfully predicted.

3D viewing

Interactive 3D view in Jmol

Sequence analysis

Secondary structure and disorder prediction

1 . . . . . . . . 10 . . . . . . . . . 20 . . . . . . . . . 30 . . . . . . . . . 40 . . . . . . . . . 50 . . . . . . . . . 60

Sequence M M H H P H P F G S N L A N S S E P Q Q P S G Q Y L N P A A D A Y N F D T F E D S D K F S P K G N V A A L R N V I H G Q

Secondary structure

SS confidence

Disorder ? ? ? ? ? ? ?
? ? ? ? ? ? ? ? ? ? ? ? ?
?
? ? ? ? ? ? ? ? ? ?
?

Disorder confidence

. . . . . . . . . 70 . . . . . . . . . 80 . . . . . . . . . 90 . . . . . . . . . 100 . . . . . . . . . 110 . . . . . . . . . 120

Sequence L D M K T P T G N S S R Y Q K P A P P P V D S T P S W A K N V K V Y E P N G Y V D P H L N K H N M G R V L D G P V N P N

Secondary structure

SS confidence

Disorder
?
?
? ? ? ? ? ? ? ?
? ? ? ? ? ? ? ? ? ? ? ? ?
?
? ?

Disorder confidence

. . . . . . . . . 130 . . . . . . . . . 140 . . . . . . . . . 150 . . . . . . . . . 160 . . . . . . . . . 170 . . . . . . . . . 180

Sequence K Y F Q G V P P A S Y S Q V K H N E S K P P V P P S T K P P H S A A Q A L Q A Q V L Q S S K A P S Q P Q Q S Q K T S Y R

Secondary structure

SS confidence

Disorder
?
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?

Disorder confidence

. . . . . . . . . 190 . . . . . . . . . 200 . . . . . . . . . 210 . . . . . . . . . 220 . . . . . . . . . 230 . . . . . . . . . 240

Sequence I P Y D V A L D P R H H L G E F D I D D S A S I I S S C I S T F G E S S E I A G F S A A A E Q R H L Y E Q Y R K K L M E

Secondary structure

SS confidence

Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?

Disorder confidence

. . . . . . . . . 250 . . . . . . . . . 260 . . . . . . . . . 270 . . . . . . . . . 280 . . . . . . . . . 290 . . . . . . . . . 300

Sequence E K N E L K E G S E T P C V S L S E K V M T S S T E N L K N G N N Q Q N Q Q P E P Q P P S S S I F N S E L T P F G H V A

Secondary structure

SS confidence

Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?

Disorder confidence

. . . . . . . . . 310 . . . . . . . . . 320 . . . . . . . . . 330 . . . . . . . . . 340 . . . . . . . . . 350 . . . . . . . . . 360

Sequence P V A K Q F E P T N F P P F S P E K E S E I K R S I D L E S S Q L L V K S K S P A P Y S T S S T D H Y G T I R R K H K P

Secondary structure

SS confidence

Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?

Disorder confidence

. . . . . . . . . 370 . . . . . . . . . 380 . . . . . . . . . 390 . . . . . . . . . 400 . . . . . . . . . 410 . . . . . . . . . 420

Sequence V A I D L S K S S P N L A S Q S P S N L F F G A S F E E K Q N R S P M T S T P S Y K E Q G F K N D S L N D S L N Q A F E

Secondary structure

SS confidence

Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?

Disorder confidence

. . . . . . . . . 430 . . . . . . . . . 440 . . . . . . . . . 450 . . . . . . . . . 460 . . . . . . . . . 470 . . . . . . . . . 480

Sequence I A S S I E T T K N A Y E A P P T P K S A S H D R S I S D T Y P V S S S T T S T W P S H T T T P T T T T A A A P I A A V

Secondary structure

SS confidence

Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?

Disorder confidence

. . . . . . . . . 490 . . . . . . . . . 500 . . . . . . . . . 510 . . . . . . . . . 520 . . . . . . . . . 530 . . . . . . . . . 540

Sequence A P Q T Y T E Q Q P M S T S M S S S V M S T N M D E P I V V G S H Q Q I P Q Q S P D S S P E R N E D M S Q W Y R K M F K

Secondary structure

SS confidence

Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?

Disorder confidence

. . . . . . . . . 550 . . . . . . . . . 560 . . . . . . . . . 570 . . . . . . . . . 580 . . . . . . . . . 590 . . . . . . . . . 600

Sequence Q M H R K G E D G S N E G K E Q H F I N P S N V T D G I G R T T P T A S N L G R S R E N L S F N Q H R P D H P S S Y F D

Secondary structure

SS confidence

Disorder
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?

Disorder confidence

. . . . . . . . . 610 . . . . . . . . . 620 . . . . . . . . . 630 . . . . . . . . . 640 . . . . . . . . . 650 . . . . . . . . . 660

Sequence S L E H G P N D Q Y N N Q E R V K Q S N E E E L L R L K A E K L A E E L R K E K E R K H S F I P S S A P S L Q N N M D R

Secondary structure

SS confidence

Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?

Disorder confidence

. . . . . . . . . 670 . . . . . . . . . 680 . . . . . . . . . 690 . . . . . . . . . 700 . . . . . . . . . 710 . . . . . . . . . 720

Sequence L N S L L Y D F S S D I Q E P A H R D Y T P Q P V M T A T A V Y K F E P R S A R E L P L N R G D I I R I I R E V D G Y W

Secondary structure

SS confidence

Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?
? ? ? ? ?

Disorder confidence

. . . . . . . . . 730 . . . . . . . . . 740 . . . . . . . . . 750 . . . . . . . . . 760 . . . . . . . . . 770 . . . . . . . . . 780

Sequence M E G E R N G R S G I F P T S Y V Q I N T G N Q G D S Q K M R A I Y P F T A R S D T E L S L K R G E I I T R R R Q I D S

Secondary structure

SS confidence

Disorder
? ?
?
? ? ?
?
? ? ? ? ?

Disorder confidence

. . . . . . . . . 790 . . . . . . . . . 800 . . . . . . . . . 810 . . . . . . . . . 820 . . . . . . . . . 830 . . . . . . . . . 840

Sequence N W L E G S N Q I G I V G I F P A S Y V E P I E Q V E Q H I P T I V P N R P K T P K I E D Q V Y N Q V Y K P N E T V I M

Secondary structure

SS confidence

Disorder
? ? ? ?
? ?
? ? ? ? ? ? ? ? ? ? ? ?
? ? ? ? ?

Disorder confidence

. . . . . . . . . 850 . . . . . . . . . 860 . . . . . . . . . 870 . . . . . . . . . 880 . . . . . . . . . 890 . . . . . . . . . 900

Sequence Q S N Q G Y Y D K A A V V P N N K V R F D L P S G S D S N L Q M S L N P H Q N Q F P P T H T Q T S T Q Q P S N Y G M K K

Secondary structure

SS confidence

Disorder ? ? ? ? ? ? ? ? ? ?
? ? ? ? ? ? ?
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?
?
? ? ? ? ? ? ? ? ? ? ?

Disorder confidence

. . . . . . . . . 910 . . . . . . . . . 920 . . . . . . . . . 930 . . . . . . . . . 940 . . . . . . . . . 950 . . . . . . . . . 960

Sequence I E Y E R E K V V E E V P P M H M D Q Y R K L N D E P K N P K K D T N I L M N A A S L I P K G S E M Y R A V Y P Y Q P Q

Secondary structure

SS confidence

Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?

Disorder confidence

. . . . . . . . . 970 . . . . . . . . . 980 . . . . . . . . . 990 . . . . . . . . . 1000 . . . . .

Sequence K E D E L Q L Y T N D I I F V V E K C D D G W F I G T S L R T G D F G I F P G N Y V K R H

Secondary structure

SS confidence

Disorder
? ?
?
? ? ?

Disorder confidence

Confidence Key
High(9) Low (0)

? Disordered ( 72%)

Alpha helix ( 11%)

Beta strand ( 9%)

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank	Aligned region

PDB 1gri chain A

Region: 686 - 1003
Aligned: 188
Modelled: 202
Confidence: 100.0%
Identity: 28%
PDB header:signal transduction adaptor
Chain: A: PDB Molecule:growth factor bound protein 2;
PDBTitle: grb2

Phyre2

PDB 2lqn chain A

Region: 740 - 1004
Aligned: 174
Modelled: 176
Confidence: 100.0%
Identity: 21%
PDB header:signaling protein
Chain: A: PDB Molecule:crk-like protein;
PDBTitle: solution structure of crkl

Phyre2

PDB 2l3s chain A

Region: 748 - 1005
Aligned: 160
Modelled: 162
Confidence: 100.0%
Identity: 19%
PDB header:structural protein
Chain: A: PDB Molecule:autoinhibited crk protein;
PDBTitle: structure of the autoinhibited crk

Phyre2

PDB 2eyz chain A

Region: 740 - 1003
Aligned: 166
Modelled: 168
Confidence: 100.0%
Identity: 17%
PDB header:signaling protein
Chain: A: PDB Molecule:v-crk sarcoma virus ct10 oncogene homolog
PDBTitle: ct10-regulated kinase isoform ii

Phyre2

PDB 1ov3 chain A

Region: 739 - 1004
Aligned: 133
Modelled: 136
Confidence: 100.0%
Identity: 27%
PDB header:oxidoreductase activator
Chain: A: PDB Molecule:neutrophil cytosol factor 1;
PDBTitle: structure of the p22phox-p47phox complex

Phyre2

PDB 1ng2 chain A

Region: 684 - 837
Aligned: 153
Modelled: 154
Confidence: 100.0%
Identity: 18%
PDB header:oxidoreductase activator
Chain: A: PDB Molecule:neutrophil cytosolic factor 1;
PDBTitle: structure of autoinhibited p47phox

Phyre2

PDB 1mv3 chain A

Region: 811 - 1004
Aligned: 194
Modelled: 194
Confidence: 99.9%
Identity: 16%
PDB header:endocytosis/exocytosis
Chain: A: PDB Molecule:myc box dependent interacting protein 1;
PDBTitle: nmr structure of the tumor suppressor bin1: alternative2 splicing in melanoma and interaction with c-myc

Phyre2

PDB 2dyb chain A

Region: 742 - 998
Aligned: 152
Modelled: 153
Confidence: 99.8%
Identity: 22%
PDB header:oxidoreductase
Chain: A: PDB Molecule:neutrophil cytosol factor 4;
PDBTitle: the crystal structure of human p40(phox)

Phyre2

PDB 2rqr chain A

Region: 914 - 1004
Aligned: 91
Modelled: 91
Confidence: 99.8%
Identity: 16%
PDB header:protein binding
Chain: A: PDB Molecule:engulfment and cell motility protein 1, linker, dedicator
PDBTitle: the solution structure of human dock2 sh3 domain - elmo1 peptide2 chimera complex

Phyre2

PDB 1ng2 chain A domain 2

Region: 938 - 1003
Aligned: 64
Modelled: 66
Confidence: 99.8%
Identity: 25%
Fold: SH3-like barrel
Superfamily: SH3-domain
Family: SH3-domain

Phyre2

PDB 2e5k chain A

Region: 935 - 1004
Aligned: 70
Modelled: 70
Confidence: 99.8%
Identity: 37%
PDB header:protein binding
Chain: A: PDB Molecule:suppressor of t-cell receptor signaling 1;
PDBTitle: solution structure of sh3 domain in suppressor of t-cell2 receptor signaling 1

Phyre2

PDB 2yt6 chain A

Region: 923 - 1004
Aligned: 82
Modelled: 82
Confidence: 99.8%
Identity: 17%
PDB header:cell cycle
Chain: A: PDB Molecule:adult male urinary bladder cdna, riken full-
PDBTitle: solution structure of the sh3_1 domain of yamaguchi sarcoma2 viral (v-yes) oncogene homolog 1

Phyre2

PDB 1udl chain A

Region: 726 - 808
Aligned: 82
Modelled: 83
Confidence: 99.8%
Identity: 28%
Fold: SH3-like barrel
Superfamily: SH3-domain
Family: SH3-domain

Phyre2

PDB 3nmz chain D

Region: 928 - 1005
Aligned: 76
Modelled: 78
Confidence: 99.8%
Identity: 18%
PDB header:cell adhesion/cell cycle
Chain: D: PDB Molecule:rho guanine nucleotide exchange factor 4;
PDBTitle: crytal structure of apc complexed with asef

Phyre2

PDB 2kym chain A

Region: 947 - 1004
Aligned: 58
Modelled: 58
Confidence: 99.8%
Identity: 24%
PDB header:signaling protein
Chain: A: PDB Molecule:bud emergence protein 1;
PDBTitle: solution structure of the bem1p sh3-ci domain from l.elongisporus in2 complex with ste20p peptide

Phyre2

PDB 2dx1 chain A

Region: 950 - 1003
Aligned: 52
Modelled: 54
Confidence: 99.7%
Identity: 23%
PDB header:signaling protein
Chain: A: PDB Molecule:rho guanine nucleotide exchange factor 4;
PDBTitle: crystal structure of rhogef protein asef

Phyre2

PDB 1pht chain A

Region: 948 - 1003
Aligned: 56
Modelled: 56
Confidence: 99.7%
Identity: 38%
Fold: SH3-like barrel
Superfamily: SH3-domain
Family: SH3-domain

Phyre2

PDB 1x6g chain A

Region: 933 - 1004
Aligned: 72
Modelled: 72
Confidence: 99.7%
Identity: 11%
PDB header:signaling protein
Chain: A: PDB Molecule:megakaryocyte-associated tyrosine-protein kinase;
PDBTitle: solution structures of the sh3 domain of human2 megakaryocyte-associated tyrosine-protein kinase.

Phyre2

PDB 2fpe chain B

Region: 947 - 1004
Aligned: 58
Modelled: 58
Confidence: 99.7%
Identity: 33%
PDB header:signaling protein
Chain: B: PDB Molecule:c-jun-amino-terminal kinase interacting protein
PDBTitle: conserved dimerization of the ib1 src-homology 3 domain

Phyre2

PDB 1bb9 chain A

Region: 940 - 1004
Aligned: 65
Modelled: 65
Confidence: 99.7%
Identity: 32%
Fold: SH3-like barrel
Superfamily: SH3-domain
Family: SH3-domain

Phyre2

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