Phyre Home Retrieve Phyre Job Id
Emaill.a.kelley@imperial.ac.uk
DescriptionA5A618
DateWed Jan 25 15:20:03 GMT 2012
Unique Job IDbf2de2b29ceddd02

Summary 

Top model
Image coloured by rainbow N → C terminus
Model (left) based on template c3nfiB_
Top template information
PDB header:dna binding protein, transcription
Chain: B: PDB Molecule:dna-directed rna polymerase i subunit rpa49;
PDBTitle: crystal structure of tandem winged helix domain of rna polymerase i2 subunit a49
Confidence and coverage
Confidence: 18.7% Coverage: 69%
20 residues ( 69% of your sequence) have been modelled with 18.7% confidence by the single highest scoring template.
You may wish to submit your sequence to Phyrealarm. This will automatically scan your sequence every week for new potential templates as they appear in the Phyre2 library.
Please note: You must be registered and logged in to use Phyrealarm.
3D viewing
Interactive 3D view in Jmol

Sequence analysis 

Secondary structure and disorder prediction 

   1........10.........20.........
Sequence  MSTDLKFSLVTTIIVLGLIVAVGLTAALH
Secondary structure 


SS confidence 




























Disorder  ???????














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???
Disorder confidence 




























 

Confidence Key
High(9)                    Low (0)
?Disordered
Alpha helix
Beta strand

Domain analysis 

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

RankAligned region

PDB 3nfi chain B

3D model

Region: 1 - 20
Aligned: 20
Modelled: 20
Confidence: 18.7%
Identity: 5%
PDB header:dna binding protein, transcription
Chain: B: PDB Molecule:dna-directed rna polymerase i subunit rpa49;
PDBTitle: crystal structure of tandem winged helix domain of rna polymerase i2 subunit a49

Phyre2

PDB 2l27 chain B

3D model

Region: 1 - 16
Aligned: 16
Modelled: 16
Confidence: 10.8%
Identity: 31%
PDB header:membrane protein, peptide binding protei
Chain: B: PDB Molecule:peptide agonist;
PDBTitle: nmr structure of the ecd1 of crf-r1 in complex with a peptide agonist

Phyre2

PDB 1q90 chain G

3D model

Region: 14 - 19
Aligned: 6
Modelled: 6
Confidence: 8.4%
Identity: 83%
PDB header:photosynthesis
Chain: G: PDB Molecule:cytochrome b6f complex subunit petg;
PDBTitle: structure of the cytochrome b6f (plastohydroquinone : plastocyanin2 oxidoreductase) from chlamydomonas reinhardtii

Phyre2

PDB 1q90 chain G

3D model

Region: 14 - 19
Aligned: 6
Modelled: 6
Confidence: 8.4%
Identity: 83%
Fold: Single transmembrane helix
Superfamily: PetG subunit of the cytochrome b6f complex
Family: PetG subunit of the cytochrome b6f complex

Phyre2
1

c3nfiB_
2

c2l27B_
3

c1q90G_
4

d1q90g_



Detailed template information 

#
Template Alignment Coverage3D Model Confidence
% i.d. Template Information
1c3nfiB_



18.7 5 PDB header:dna binding protein, transcription
Chain: B: PDB Molecule:dna-directed rna polymerase i subunit rpa49;
PDBTitle: crystal structure of tandem winged helix domain of rna polymerase i2 subunit a49
2c2l27B_



10.8 31 PDB header:membrane protein, peptide binding protei
Chain: B: PDB Molecule:peptide agonist;
PDBTitle: nmr structure of the ecd1 of crf-r1 in complex with a peptide agonist
3c1q90G_



8.4 83 PDB header:photosynthesis
Chain: G: PDB Molecule:cytochrome b6f complex subunit petg;
PDBTitle: structure of the cytochrome b6f (plastohydroquinone : plastocyanin2 oxidoreductase) from chlamydomonas reinhardtii
4d1q90g_



8.4 83 Fold:Single transmembrane helix
Superfamily:PetG subunit of the cytochrome b6f complex
Family:PetG subunit of the cytochrome b6f complex

Binding site prediction 

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction 

Transmembrane helices have been predicted in your sequence to adopt the topology shown below



Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
 
© Structural Bioinformatics Group
Imperial College London
Lawrence Kelley, Benjamin Jefferys
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Terms and Conditions
Component software
Template detection: HHpred 1.51
Secondary structure prediction: Psi-pred 2.5
Disorder prediction: Disopred 2.4
Transmembrane prediction: Memsat_SVM
Multi-template modelling and ab initio: Poing 1.0