Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	R	L	H	V	K	L	K	E	F	L	S	M	F	F	M	A	I	L	F	F	P	A	F	N	A	S	L	F	F	T	G	V	K	P	L	Y	S	I	I	K	C	S	T	E	I	F	Y	D	W	R	M	L	I	L	C	F	G	F	M
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		S	F	S	F	L	N	I	H	V	I	L	L	T	I	I	K	S	F	L	I	K	K	T	K	V	V	N	F	A	T	D	I	T	I	Q	L	T	L	I	F	L	L	I	A	I	V	I	A	P	L	I	A	P	F	V	T	G	Y	V	N
Secondary structure
SS confidence
Disorder																					?	?			?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.
Sequence		T	N	Y	H	P	C	G	N	N	T	G	I	F	P	G	A	I	Y	I	K	N	G	M	K	C	N	N	G	Y	I	S	R	K	E	D	S	A	V	K
Secondary structure
SS confidence
Disorder																											?	?	?					?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3izc chain I Region: 74 - 93 Aligned: 20 Modelled: 20 Confidence: 46.1% Identity: 35% PDB header:ribosome Chain: I: PDB Molecule:60s ribosomal protein rpl10 (l10e); PDBTitle: localization of the large subunit ribosomal proteins into a 6.1 a2 cryo-em map of saccharomyces cerevisiae translating 80s ribosome Phyre2 PDB 3iz5 chain I Region: 74 - 92 Aligned: 19 Modelled: 19 Confidence: 36.8% Identity: 37% PDB header:ribosome Chain: I: PDB Molecule:60s ribosomal protein l10 (l10e); PDBTitle: localization of the large subunit ribosomal proteins into a 5.5 a2 cryo-em map of triticum aestivum translating 80s ribosome Phyre2 PDB 4a19 chain L Region: 74 - 88 Aligned: 15 Modelled: 15 Confidence: 32.2% Identity: 40% PDB header:ribosome Chain: L: PDB Molecule:rpl34; PDBTitle: t.thermophila 60s ribosomal subunit in complex with2 initiation factor 6. this file contains 26s rrna and3 proteins of molecule 2. Phyre2 PDB 2xcq chain A Region: 115 - 130 Aligned: 16 Modelled: 16 Confidence: 28.0% Identity: 38% PDB header:isomerase Chain: A: PDB Molecule:dna gyrase subunit b, dna gyrase subunit a; PDBTitle: the 2.98a crystal structure of the catalytic core (b'a'2 region) of staphylococcus aureus dna gyrase Phyre2 PDB 2xcs chain D Region: 115 - 130 Aligned: 16 Modelled: 16 Confidence: 26.7% Identity: 38% PDB header:isomerase Chain: D: PDB Molecule:dna gyrase subunit b, dna gyrase subunit a; PDBTitle: the 2.1a crystal structure of s. aureus gyrase complex with gsk2994232 and dna Phyre2 PDB 1zps chain A domain 1 Region: 119 - 151 Aligned: 30 Modelled: 33 Confidence: 23.6% Identity: 23% Fold: HisI-like Superfamily: HisI-like Family: HisI-like Phyre2 PDB 1gpe chain A domain 2 Region: 114 - 127 Aligned: 14 Modelled: 14 Confidence: 22.1% Identity: 21% Fold: FAD-linked reductases, C-terminal domain Superfamily: FAD-linked reductases, C-terminal domain Family: GMC oxidoreductases Phyre2 PDB 2wl2 chain B Region: 115 - 130 Aligned: 16 Modelled: 16 Confidence: 22.1% Identity: 44% PDB header:isomerase Chain: B: PDB Molecule:dna gyrase subunit a; PDBTitle: crystal structure of n-terminal domain of gyra with the2 antibiotic simocyclinone d8 Phyre2 PDB 1mv3 chain A Region: 126 - 140 Aligned: 14 Modelled: 15 Confidence: 21.6% Identity: 21% PDB header:endocytosis/exocytosis Chain: A: PDB Molecule:myc box dependent interacting protein 1; PDBTitle: nmr structure of the tumor suppressor bin1: alternative2 splicing in melanoma and interaction with c-myc Phyre2 PDB 1qhm chain A Region: 115 - 136 Aligned: 22 Modelled: 22 Confidence: 20.0% Identity: 27% Fold: PFL-like glycyl radical enzymes Superfamily: PFL-like glycyl radical enzymes Family: PFL-like Phyre2 PDB 1h16 chain A Region: 115 - 136 Aligned: 22 Modelled: 22 Confidence: 19.3% Identity: 27% Fold: PFL-like glycyl radical enzymes Superfamily: PFL-like glycyl radical enzymes Family: PFL-like Phyre2 PDB 1cf3 chain A domain 2 Region: 114 - 127 Aligned: 14 Modelled: 14 Confidence: 17.9% Identity: 29% Fold: FAD-linked reductases, C-terminal domain Superfamily: FAD-linked reductases, C-terminal domain Family: GMC oxidoreductases Phyre2 PDB 2kl7 chain A Region: 140 - 149 Aligned: 10 Modelled: 10 Confidence: 15.5% Identity: 60% PDB header:signaling protein, structural protein Chain: A: PDB Molecule:fibulin-4; PDBTitle: solution nmr structure of the egf-like 1 domain of human2 fibulin-4. northeast structural genomics target hr6275 Phyre2 PDB 1ab4 chain A Region: 115 - 130 Aligned: 16 Modelled: 16 Confidence: 15.3% Identity: 44% Fold: Type II DNA topoisomerase Superfamily: Type II DNA topoisomerase Family: Type II DNA topoisomerase Phyre2 PDB 1wkw chain B Region: 42 - 56 Aligned: 15 Modelled: 15 Confidence: 14.9% Identity: 40% PDB header:translation/protein binding Chain: B: PDB Molecule:eukaryotic translation initiation factor 4e PDBTitle: crystal structure of the ternary complex of eif4e-m7gpppa-2 4ebp1 peptide Phyre2 PDB 1p9j chain A Region: 119 - 147 Aligned: 24 Modelled: 29 Confidence: 13.1% Identity: 17% Fold: Knottins (small inhibitors, toxins, lectins) Superfamily: EGF/Laminin Family: EGF-type module Phyre2 PDB 2awi chain A domain 1 Region: 122 - 140 Aligned: 19 Modelled: 19 Confidence: 11.9% Identity: 42% Fold: lambda repressor-like DNA-binding domains Superfamily: lambda repressor-like DNA-binding domains Family: PrgX N-terminal domain-like Phyre2 PDB 2nov chain D Region: 115 - 130 Aligned: 16 Modelled: 16 Confidence: 11.4% Identity: 31% PDB header:isomerase Chain: D: PDB Molecule:dna topoisomerase 4 subunit a; PDBTitle: breakage-reunion domain of s.pneumoniae topo iv: crystal2 structure of a gram-positive quinolone target Phyre2 PDB 2jxb chain A Region: 126 - 140 Aligned: 14 Modelled: 15 Confidence: 10.4% Identity: 36% PDB header:signaling protein complex Chain: A: PDB Molecule:t-cell surface glycoprotein cd3 epsilon chain, PDBTitle: structure of cd3epsilon-nck2 first sh3 domain complex Phyre2 PDB 2dlp chain A Region: 126 - 140 Aligned: 14 Modelled: 15 Confidence: 10.3% Identity: 36% PDB header:structural protein Chain: A: PDB Molecule:kiaa1783 protein; PDBTitle: solution structure of the sh3 domain of human kiaa17832 protein Phyre2 1 c3izci_ 2 c3iz5i_ 3 c4a19L_ 4 c2xcqA_ 5 c2xcsD_ 6 d1zpsa1 7 d1gpea2 8 c2wl2B_ 9 c1mv3A_ 10 d1qhma_ 11 d1h16a_ 12 d1cf3a2 13 c2kl7A_ 14 d1ab4a_ 15 c1wkwB_ 16 d1p9ja_ 17 d2awia1 18 c2novD_ 19 c2jxbA_ 20 c2dlpA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3izci_ 46.1 35 PDB header:ribosome Chain: I: PDB Molecule:60s ribosomal protein rpl10 (l10e); PDBTitle: localization of the large subunit ribosomal proteins into a 6.1 a2 cryo-em map of saccharomyces cerevisiae translating 80s ribosome 2 c3iz5i_ 36.8 37 PDB header:ribosome Chain: I: PDB Molecule:60s ribosomal protein l10 (l10e); PDBTitle: localization of the large subunit ribosomal proteins into a 5.5 a2 cryo-em map of triticum aestivum translating 80s ribosome 3 c4a19L_ 32.2 40 PDB header:ribosome Chain: L: PDB Molecule:rpl34; PDBTitle: t.thermophila 60s ribosomal subunit in complex with2 initiation factor 6. this file contains 26s rrna and3 proteins of molecule 2. 4 c2xcqA_ 28.0 38 PDB header:isomerase Chain: A: PDB Molecule:dna gyrase subunit b, dna gyrase subunit a; PDBTitle: the 2.98a crystal structure of the catalytic core (b'a'2 region) of staphylococcus aureus dna gyrase 5 c2xcsD_ 26.7 38 PDB header:isomerase Chain: D: PDB Molecule:dna gyrase subunit b, dna gyrase subunit a; PDBTitle: the 2.1a crystal structure of s. aureus gyrase complex with gsk2994232 and dna 6 d1zpsa1 23.6 23 Fold:HisI-like Superfamily:HisI-like Family:HisI-like 7 d1gpea2 22.1 21 Fold:FAD-linked reductases, C-terminal domain Superfamily:FAD-linked reductases, C-terminal domain Family:GMC oxidoreductases 8 c2wl2B_ 22.1 44 PDB header:isomerase Chain: B: PDB Molecule:dna gyrase subunit a; PDBTitle: crystal structure of n-terminal domain of gyra with the2 antibiotic simocyclinone d8 9 c1mv3A_ 21.6 21 PDB header:endocytosis/exocytosis Chain: A: PDB Molecule:myc box dependent interacting protein 1; PDBTitle: nmr structure of the tumor suppressor bin1: alternative2 splicing in melanoma and interaction with c-myc 10 d1qhma_ 20.0 27 Fold:PFL-like glycyl radical enzymes Superfamily:PFL-like glycyl radical enzymes Family:PFL-like 11 d1h16a_ 19.3 27 Fold:PFL-like glycyl radical enzymes Superfamily:PFL-like glycyl radical enzymes Family:PFL-like 12 d1cf3a2 17.9 29 Fold:FAD-linked reductases, C-terminal domain Superfamily:FAD-linked reductases, C-terminal domain Family:GMC oxidoreductases 13 c2kl7A_ 15.5 60 PDB header:signaling protein, structural protein Chain: A: PDB Molecule:fibulin-4; PDBTitle: solution nmr structure of the egf-like 1 domain of human2 fibulin-4. northeast structural genomics target hr6275 14 d1ab4a_ 15.3 44 Fold:Type II DNA topoisomerase Superfamily:Type II DNA topoisomerase Family:Type II DNA topoisomerase 15 c1wkwB_ 14.9 40 PDB header:translation/protein binding Chain: B: PDB Molecule:eukaryotic translation initiation factor 4e PDBTitle: crystal structure of the ternary complex of eif4e-m7gpppa-2 4ebp1 peptide 16 d1p9ja_ 13.1 17 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:EGF/Laminin Family:EGF-type module 17 d2awia1 11.9 42 Fold:lambda repressor-like DNA-binding domains Superfamily:lambda repressor-like DNA-binding domains Family:PrgX N-terminal domain-like 18 c2novD_ 11.4 31 PDB header:isomerase Chain: D: PDB Molecule:dna topoisomerase 4 subunit a; PDBTitle: breakage-reunion domain of s.pneumoniae topo iv: crystal2 structure of a gram-positive quinolone target 19 c2jxbA_ 10.4 36 PDB header:signaling protein complex Chain: A: PDB Molecule:t-cell surface glycoprotein cd3 epsilon chain, PDBTitle: structure of cd3epsilon-nck2 first sh3 domain complex 20 c2dlpA_ 10.3 36 PDB header:structural protein Chain: A: PDB Molecule:kiaa1783 protein; PDBTitle: solution structure of the sh3 domain of human kiaa17832 protein 21 d1hlya_ not modelled 9.6 60 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Scorpion toxin-like Family:Short-chain scorpion toxins 22 d1vh4a_ not modelled 9.1 15 Fold:Single-stranded right-handed beta-helix Superfamily:Stabilizer of iron transporter SufD Family:Stabilizer of iron transporter SufD 23 d1awwa_ not modelled 8.7 21 Fold:SH3-like barrel Superfamily:SH3-domain Family:SH3-domain 24 d1qlya_ not modelled 7.7 21 Fold:SH3-like barrel Superfamily:SH3-domain Family:SH3-domain 25 d1ju2a2 not modelled 7.6 22 Fold:FAD-linked reductases, C-terminal domain Superfamily:FAD-linked reductases, C-terminal domain Family:GMC oxidoreductases 26 c1jdmA_ not modelled 7.5 41 PDB header:membrane protein Chain: A: PDB Molecule:sarcolipin; PDBTitle: nmr structure of sarcolipin 27 c3u7xC_ not modelled 7.2 46 PDB header:translation Chain: C: PDB Molecule:eukaryotic translation initiation factor 4e-binding protein PDBTitle: crystal structure of the human eif4e-4ebp1 peptide complex without cap 28 c3smuC_ not modelled 7.2 46 PDB header:translation Chain: C: PDB Molecule:eukaryotic translation initiation factor 4e-binding protein PDBTitle: crystal structure of the human eif4e-4ebp1 peptide complex without cap 29 c3u7xD_ not modelled 7.2 46 PDB header:translation Chain: D: PDB Molecule:eukaryotic translation initiation factor 4e-binding protein PDBTitle: crystal structure of the human eif4e-4ebp1 peptide complex without cap 30 c3smuD_ not modelled 7.2 46 PDB header:translation Chain: D: PDB Molecule:eukaryotic translation initiation factor 4e-binding protein PDBTitle: crystal structure of the human eif4e-4ebp1 peptide complex without cap 31 c1x6bA_ not modelled 7.2 24 PDB header:signaling protein Chain: A: PDB Molecule:rho guanine exchange factor (gef) 16; PDBTitle: solution structures of the sh3 domain of human rho guanine2 exchange factor (gef) 16 32 c2j06B_ not modelled 6.3 6 PDB header:signal transduction Chain: B: PDB Molecule:ras gtpase-activating protein 1; PDBTitle: crystal structure of the rasgap sh3 domain at 1.8 angstrom2 resolution 33 c2a28D_ not modelled 6.0 43 PDB header:signaling protein Chain: D: PDB Molecule:bzz1 protein; PDBTitle: atomic-resolution crystal structure of the second sh32 domain of yeast bzz1 determined from a pseudomerohedrally3 twinned crystal 34 d1wt8a1 not modelled 6.0 63 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Scorpion toxin-like Family:Short-chain scorpion toxins 35 c2oa9B_ not modelled 6.0 30 PDB header:hydrolase Chain: B: PDB Molecule:r.mvai; PDBTitle: restriction endonuclease mvai in the absence of dna 36 c3n7sB_ not modelled 5.9 56 PDB header:membrane protein Chain: B: PDB Molecule:calcitonin gene-related peptide type 1 receptor; PDBTitle: crystal structure of the ectodomain complex of the cgrp receptor, a2 class-b gpcr, reveals the site of drug antagonism 37 d2hf5a1 not modelled 5.6 67 Fold:EF Hand-like Superfamily:EF-hand Family:Calmodulin-like 38 c2drmB_ not modelled 5.6 29 PDB header:contractile protein Chain: B: PDB Molecule:acanthamoeba myosin ib; PDBTitle: acanthamoeba myosin i sh3 domain bound to acan125 39 c2kdcC_ not modelled 5.6 13 PDB header:transferase Chain: C: PDB Molecule:diacylglycerol kinase; PDBTitle: nmr solution structure of e. coli diacylglycerol kinase2 (dagk) in dpc micelles 40 d2dmda2 not modelled 5.5 28 Fold:beta-beta-alpha zinc fingers Superfamily:beta-beta-alpha zinc fingers Family:Classic zinc finger, C2H2 41 c2axkA_ not modelled 5.4 55 PDB header:toxin Chain: A: PDB Molecule:discrepin; PDBTitle: solution structure of discrepin, a scorpion venom toxin2 blocking k+ channels. 42 c1griA_ not modelled 5.1 43 PDB header:signal transduction adaptor Chain: A: PDB Molecule:growth factor bound protein 2; PDBTitle: grb2

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0