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ANALYSIS FOR 3-ISOTHIO-CYANATO-4-IODOBENZENE SULPHON [ILI]
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Formula : C7,H4,S2,O3,N1,I1
Solution chemistry: [C6,H3]1,[S1,O3]1,[N1,C1,S1]1,[I]1 -1
All distances below are to the element I [ iodine ]
| PROTEIN: 1PTE D-ALANYL-CARBOXYPEPTIDASE-TRANSPEPTIDASE
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Hev Atom conc.: 10.000 mM
Precip & conc.: 30.000 % PEG 8000
Buffer & conc.: 0.050 M PHOSPHATE
Addict & conc.:
Comments : CRYSTAL WASHED 8 HOURS WITH REAGENT FREE PEG SOLUT
Reference : KELLY et.al. 1982 :1985 :
pH 6.8 Soak time = 72.00 Hours Reference number = 95.03
Site 1: 28 SER CA 4.87 ( 4.87) . . .
: 29 ALA CA 4.61 ( 4.61) . . .
: 30 LYS CA 4.13 ( 4.13) . . .
: 31 GLY CA 4.96 ( 4.96) . . .
: 35 VAL CA 5.77 ( 5.77) . . .
: 72 TYR CA 4.81 ( 4.81) . . .
: 73 GLY CA 2.96 ( 2.96) . . .
: 74 GLY CA 5.97 ( 5.97) . . .
Site 2: 34 GLY CA 5.08 ( 5.08) . . .
: 35 VAL CA 5.03 ( 5.03) . . .
: 37 SER CA 4.62 ( 4.62) . . .
: 156 GLY CA 4.46 ( 4.46) . . E
: 157 SER CA 5.58 ( 5.58) . . .
: 313 GLY CA 5.38 ( 5.38) . . .
: 319 GLY CA 4.66 ( 4.66) . . .
: 338D ALA CA 5.51 ( 5.51) . . .
: 72 TYR CA 5.10 ( 5.10) . . .
| PROTEIN: 2CNA CONCANAVALIN A
|
Bound Ligands : MN MANGANESE ION: CA CALCIUM ION:
Hev Atom conc.: 1.000 mM
Precip & conc.: 0.100 M SODIUM SULPHATE
Buffer & conc.: 0.010 M SODIUM MALEATE
Addict & conc.:
Comments : PROTEIN PREPARED BY SUMNER AND HOWELL (4)
Reference : BECKER et.al. 1975 :QUIOCHO et.al. 1971 :
pH 6.8 Soak time = 1104.00 Hours Reference number = 134.06
Site 1: 81 LEU CD2 5.82 ( 8.80) . . T
: 85 LEU CD1 4.66 ( 8.35) . . T
: 89 VAL CG1 3.64 ( 6.20) . . E
: 90 ARG O 5.99 ( 6.94) . . E
: 91 VAL CG2 4.49 ( 6.60) . . E
: 111 PHE CE1 4.84 ( 9.32) . . E
: 113 SER CB 5.89 ( 7.24) . . E
: 179 VAL CG1 3.67 ( 6.02) . . E
: 181 ILE CD1 3.22 ( 5.88) . . E
: 191 PHE CE1 3.86 ( 6.02) . . E
: 214 ILE CD1 2.96 ( 5.95) . . E